Yes Anupre has two Prems the first one died of choloform overdose which Komolika and Subrato were behind.
The second Prem was born because Sneha (Anupre's daughter) had bone marrow cancer and needed a bone marrow which could only come from a sibling so to save her Anupre had the second Prem. At that time Prerna-Bajaj were in a live in relationship and Anurag-Aparna were married.